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The proposed method enables a new stability lobes diagram to be established that takes into account the effect of spindle speed on dynamic behaviour.
The experiments were conducted to study the effect of spindle speed and feed rate on feed force, surface finish and burr height using solid carbide multifacet drills of 5 mm diameter.
The response surface plots indicate that the effect of spindle speed and feed rate is less significant at low depth of cut but this effect significantly increases the residual stress, cutting force and surface roughness as the depth of cut increases.
We believe that the assay of symmetric stem cell division is the best readout to examine the direct effect of spindle orientation defect.
Although no drastic effect of functionally inactive MGRN1 is obvious on the mitotic index or the completion of mitosis in the short term (Supplementary Figure S1E), the effect of spindle misorientation had a cumulative detrimental effect over multiple cycles of cell division.
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In addition, the effect of spindles on plasticity also depends on the presence of slow oscillations59.
The effects of spindle error motion, spindle unbalance induced eccentricity, thermal error and position drift of AAL are analyzed.
The effects of spindle speed, feed per tooth and depth of cut on tool wear, force and surface roughness were investigated.
Altogether, data presented in Figures 1, 2, 3 indicate that EBV rendered the TP53-mutated BL41 cell line sensitive to cytotoxic effects of spindle poisons.
Possible effects of spindle movement during imaging on calculated particle velocities were analyzed and found to be negligible; corrections for spindle movement were therefore not included in the final data analysis.
In mitosis, exogenous expression of eGFP HDAC3 had a small effect on spindle formation and chromosomal organization, and acetylated tubulin was still observed in the spindle microtubules.
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