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There was no effect of ND on mean tail moment (MTM) before ENU treatment, or on the development of strand breaks between 0 and 8 h after ENU.
The independent effect of ND on 90 days functional outcome and long-term mortality was assessed using logistic regression (good 90-day outcome) and Cox Proportional Hazard Model (long-term mortality).
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Therefore, whereas the effects of ND on differentiation are mediated by mTOR, its effects on proliferation are likely to be mediated by additional cellular mechanisms.
To test whether mTOR regulates the effects of ND on the CMZ, we first immunostained for phospho-Ribosomal protein S6 (pS6), the cardinal marker of mTOR activity.
To test whether the effects of ND on the CMZ were specific to Xenopus, we also deprived zebrafish larvae of nutrients by removing the majority of their yolk at 56 hpf.
To investigate the effects of ND on the proliferation-differentiation programme, we used amphibian and fish retinas, which grow throughout life by the addition of new cells from the ciliary marginal zone (CMZ), a neurogenic niche at the retina periphery (supplementary material Fig. S1A) (reviewed by Agathocleous and Harris, 2009).
The actual effect of ND care on clinical outcomes should be the focus of future trials.
Meanwhile, with increasing of the dose of oMWCNTs from 0 to 500 μg, the oMWCNTs could improve the tissue uptakes of NDs (Figure 7). Figure 6 Effect of NDs doses on biodistribution of 500 μg 99m Tc-oMWCNTs in mice 2 h after co-injection.
On the other hand, if the effect of ND is major, Fig. 3 should show similar curves.
The effects of Nd content on the phase structure of the ceramics were investigated.
In this study, we investigate the effects of composition and structural characteristics of ND on the oxidation kinetics.
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