It comprises a novel tool for analyzing proteins and protein models, for simulating the effect of mutations under evolutionary processes, and a quantitative description of mutational robustness.
Using the same mutational strategy, mutations were introduced into the ERev protein and the effect of mutations was evaluated in a Rev nuclear export activity assay (Fig 4A).
Open image in new window Figure 6 Effect of mutations on the transport activity of hENT1.
The team then tallied the effect of mutations on the ribozymes' shape and cleaving ability.
Our work also sheds light into the effect of mutations and external conditions, such as pH, on the FP activity.
Allele mining identifies superior alleles from related genotypes that may have been the effect of mutations in the process of evolution.
New deep-sequencing and microfluidics technologies allow us to quantify the effect of mutations and trace the evolution of viral populations with single-genome and single-nucleotide resolution.
Similarly, we tested the effect of mutations in hASF1a and hASF1b on protein stability.
Rather we were interested in the general effect of mutations in the sequences.
This supports, albeit indirectly, a deleterious effect of mutations in these regions of the protein.
This method allows to quantitatively measure the effect of mutations and aging on worm's crawling velocity.
