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Figure 5 Effect of inoculum size on laccase production by Serratia marcescens at 25°C.
Attempts have also been made to determine the effect of inoculum size and type on the process of pellet formation at acidic pH conditions.
Krishna (1999) studied the effect of inoculum size (5 40 %) on production of exoglucanase and endoglucanase by B. subtilis and revealed that 15%% (v/w) of inoculum size was optimal for the production of both enzymes.
Our results show that the observed effect of inoculum size from the previous report at a single temperature is not restricted to a specific growth condition, but rather a general phenomenon.
Table 2 Effect of inoculum size on P. indica biomass immobilized on different amounts of 0.5 cm3 foam cubes PUF (g flask−1) Immobilized biomass (mg flask−1) Inoculum size (ml spore/mycelium suspension) 0.5 1.0 2.0 5.0 0.2 210 365 433 454* 0.5 377 515 619 623 1.0 400 561 640 650 Values are means of three replicates ± standard deviation * Standard errors are smaller than 5% and are not shown.
Therefore, each selected organic acid was added to the medium at its IC50 concentration to examine the effect of inoculum size on the inhibition of the organic acid.
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The pH and temperature value of the cultivation medium were the most significant variables and the effects of inoculum size and agitation speed were slightly lower.
The effects of inoculum size, temperature or initial pH on the potency of organic acids were examined using acid concentrations of IC50.
The analysis of the empirical model showed that linear effect of inoculums size with quadratic effect of pH and inoculum size influenced butanol production at 99% probability level (P < 0.01).
The number of colony-forming units (CFU/mL) dropped rapidly in untreated liquid fractions equivalent to 14% DM content, independently of inoculum size, showing that the toxic compounds had a fatal effect on the fermenting microorganism.
With the increase in inoculum size the biomass concentration increases, reaches a maximum and then it declines with the increase of inoculum size.
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