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The MANOVA with repeated measure was employed to determine the main and interaction effect of GL on test-time and achievement.
Initially, the colonogenic assay was used to determine the effect of GL on cell viability.
Similarly, no effect of GL on optimal codon usage was found in various species of Populus (Ingvarsson 2008).
However, the effect of GL on HMGB1 expression in endotoxemia as well as its underlying molecular mechanism remained unclear.
There was no effect of GL on mean HOMA-IR or on mean integrated post-prandial concentrations of glucose-adjusted insulin, IGF-1 or IGFBP-3.
The results indicate that the anti-cancer effect of GL on breast cancer cells is most likely attributable to z-Gug as well as other constituent(s).
Similar(52)
To study the effects of GL on brain, determination of an appropriate GL condition for animals is needed.
Experiments to determine the effects of GL on β-catenin level were carried out using the Surveyor™ IC Human total β-Catenin Immunoassay kit (R&D Systems) following the manufacturer's instructions.
However, growth inhibitory effect of GL to the cancer cells was ~10-fold stronger compared with that of z-Gug.
In our present studies, we were the first to report the anti-cancer effect and mechanism of GL on human breast cancer cells.
The effect of GL and z-Gug on cell viability was determined by the colonogenic survival assay and trypan blue dye exclusion assays as described by us previously [ 13, 25].
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