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Furthermore, we demonstrate a direct, GR-dependent effect of GCs on NSC differentiation into oligodendrocytes, which involves an upregulation of pro-oligodendrogenic genes, and increased nuclear localization of Olig1 protein.
In this experiment, PC axons did not change significantly their growth pattern (Fig. 4E, E17+GCflipflip vs E17, Chi square or Fisher exact tests always P>0.30), showing that the main effect of GCs on the growth mode of PC axons is exerted through contact-mediated factors, while high KCl concentration has no overt influence.
In this study we investigate the effect of GCs on mRNA translation on a genome-wide scale.
The effect of GCs on lymphoid cells is dramatic and includes G1-phase cell-cycle arrest and apoptosis.
Another direct effect of GCs on osteocytes is the induction of apoptosis through activation of caspase 3 [ 34].
More recently, a diminished inhibitory effect of GCs on PBMC proliferation in vitro was shown in a larger cohort of GC resistant RA patients [ 3].
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The effect of GC on cytokine production, either pro or anti-inflammatory, is dependent on both the dose and duration of GC exposure.
We also investigated the effect of GC on epithelial mesenchymal transition-related markers.
Surprisingly, previous results came as the negative effect of GC on fibroblasts proliferation and the ability of synthesizing mature collagen.
To reinforce the effect of GC on TGF- β1 production, we detected TGF- β1 production in human liver cell line Chang Liver after cortisol treatment.
The ANCOVA examined the effect of GC on genetic distance, with sequence type as a factor to determine if GC content alone accounted for the differences in genetic distance between our coding and intergenic data.
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