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Therefore, we evaluated the effect of EMB on these pathways using western blot analysis.
The accumulated evidence indicates that the cytotoxic effect of EMB on the retinal cells might be associated with autophagy dysfunction.
In conclusion, monitoring of multiple microcolonies allows rapid determination of EMB susceptibility and has applications in diagnostics and for characterization of the inhibitory effect of EMB on strains carrying resistance associated mutations.
Addition of the EmbA, EmbB and EmbC inhibitor ethambutol (EMB), resulted in the loss of the upper band with only the lower band remaining, this being due to the noninhibitory effect of EMB on the terminal β(1 → 2) capping enzyme, AftB (Seidel et al. 2007; Figure 4, lane 3).
To determine the effect of EMB on autophagy, we investigated the protein levels of class III phosphoinositide 3-kinase (PI3C3; also known as Vps34), beclin-1, p62 and microtubule-associated protein I light chain 3 (LC3) in the retinas of rats treated with EMB or PBS by intraperitoneal (IP) injection.
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Probes with a significant main effect of EMB concentration were fold-change-filtered against the 0 ppb control (FC ≥ 1.5).
In addition to being investigated for effects of EMB, the data from experiment 2 were also analysed with respect to differences in constitutive transcript expression between strains.
To further investigate the effects of EMB exposure, a list of those features that were significantly affected by EMB treatment or for which significant interactions between treatment and other factors were observed (treatment × strain; treatment × time; treatment × strain × time) was compiled.
To analyse the effect of direct inhibition of Emb protein activity on pri-miRNA level, we treated 8 × 10 S2R+ cells with 25 ng/ml LMB (Sigma) or vehicle control (70% methanol) in 12-well plates for 2 h.
Describes the effect of "emulsions" on pasta.
Another possible explanation for the lack of correlation between the embB gene alterations and EMB resistance may relate to a cumulative effect of multiple mutations on the development of EMB resistance.
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