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Conventional wisdom in the control of malaria vector mosquitoes is that a repellent action will neutralize the toxic effect of a compound and thus reduce the effectiveness of the chemical.
The effect of a compound on the entire network, and thus, in this particular case, the production of PGE2, was assessed by using the network connectivity measures (network efficiency and network flux).
In most anti-infective drug discovery cascade bactericidal effect of a compound is rarely monitored.
We next tested the in vivo effect of a compound CV2 mutant version that lacks both BMP and Chordin binding.
To probe the effect of a compound on MT stability the molecule was added 5 min after initiation of tubulin assembly.
Therefore, unlike the classical diffusion assay which reports on the absolute antimicrobial effect of a compound, the assay we developed is sensitive to the differential effect of compounds on competing microbial strains.
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Our study suggests that the high throughput approach is an effective tool when no prior knowledge is established for the biological effects of a compound.
Nonetheless, the effects of a compound at the cellular or the organism level are poorly understood in this case, as these methods cannot account for the interactions of a compound with other unrelated targets, which are not captured in the PCM model.
With the development of pharmacology, more and more attention has been paid to chemogenomics [15], a discipline that tries to understand the global effects of a compound in a complete biological system.
The balance between the therapeutic and toxicological effects of a compound is a very important measure of the usefulness of a pharmacological drug.
Simultaneously testing multiple Th lineages and drug concentrations allows a more complete characterization of the effects of a compound on Th differentiation and furthers mechanistic conclusions.
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