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Essential revisions: 1) The manuscript examines the effect of TERT promoter mutations with respect to telomerase activity and telomere length.
Essential revisions: 1) The issue requires great clarity and consistency of definitions.
This issue was addressed in our response to Essential revisions above.
Essential revisions: 1) A key issue is that the authors use different cell types to come to their conclusions.
Essential revisions: 1) Figure 2: the first experiment in the manuscript tests the effect of replacing phenylalanine residues in the TM span with leucine or alanine residues.
Essential revisions: 1) It is standard in studies using siRNAs or shRNAs to rule out off target effects.
Essential revisions: 1) The authors discovered that knocking down the same genes in different isolates has different effects on embryonic lethality and the authors state that this observation indicates that there is cryptic genetic variation.
Essential revisions are below.
Essential revisions: 1) The dose response analysis needs revision.
Two textual revisions are considered essential for publication, highlighted as #1 and #2 under "Essential revisions".
Essential revisions: 1) Better define darting and darters.
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