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The noncancer health effect endpoints themselves vary in severity.
Ignoring information about the inter-relationships between beneficial and 'side effect' endpoints in such instances may have implications for quantifying the benefits associated with treatment.
However, ecotoxicological research frequently focuses only on easily measurable endpoints, typically mortality, although more sensitive tests on effect endpoints such as reproduction and growth are also used widely.
The reasons for the discrepancy of detected effect level are not clear, and the impacting factors may include cross-species, different exposure window during life course, different exposure route and/or duration to BPA for animal studies and different cell types or effect endpoints tested for cell experiments.
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Generally, deviations from an additivity expectation could be explained due to experimental factors, specific limitations of the effect endpoint or masking side effects such as cytotoxicity in in vitro assays.
Note that in this step uncertainties are are with respect to the same magnitude of the same effect (endpoint).
The experts further suggested that more health-effect endpoints were included in the toxicology part, as well as food and biota in the environmental matrix part.
RSA potentiated online congruence effects (intermediate states of behavior) but not offline congruence effects (endpoint states of behavior).
Regardless, mean qPCR knockdown did not qualitatively predict either degree or direction of alterations in either respiratory capacity or anesthetic sensitivity (Table 3 and Figure S1), indicating that not all subunits equally effect these endpoints.
However, the test is expensive and labor intensive, requiring manual reading and interpretation of virus induced cytopathic effect (CPE) endpoints.
As these agents may have more of a cytostatic than cytotoxic effect, alternative endpoints to objective response may be appropriate.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com