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A position effect assay for boundaries of higher order chromosomal domains.
In a cytopathic effect assay, SA-2 dose dependently inhibited H1N1, H3N2 and the oseltamivir-resistant mutant H1N1-H275Y influenza viruses in both virus-infected MDCK and A549 cells, with 50% effective concentrations (EC50) in MDCK cells of 9.6, 19.2 and 19.8 μM respectively, and 50% cytotoxic concentration (CC50) of 444.5 μM, showing competitive antiviral activity with oseltamivir in vitro.
Salmonella typhimurium (S. typhimurium SL1344) and Staphylococcus aureus (S. aureus) (ATCC 25923) were used for the antibacterial effect assay.
Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 25923), Micrococcus luteus(ATCC 15307) and Pseudomonas Afruginosa (ATCC 27853) were used for the antibacterial effect assay.
Titration was carried out by end-point dilution, cytopathic effect assay, with centrifugation of infected 96-well plates as described in detail by Nyberg-Hoffman et al [40].
Titers of virus stocks were determined by endpoint cytopathic effect assay with the following modifications (17).
Similar(45)
These tests are to be complemented by a suite of biological effect assays.
Yield reduction assays (YRA), plaque reduction assays (PRA), and cytopathic effect assays (CPE) are common cell culture viral assays.
Luciferase assays, co-immunoprecipitation and Western blotting, immunofluorescent cell staining and cytopathic effect assays were used to characterize the E1A-HD2 fusion protein and virus in vitro.
Despite showing five-fold more activity in cytopathic effect assays in vitro, the activity of the virus was unchanged in xenografts of SW620 colon cancer cells in vivo.
In side effect assays where comparisons showed a significant overall p-value, a Student's t-test was included between prednisolone and vehicle groups for normally distributed measurements.
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