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The editing rate for each sample (circle) is presented along with the center value (mean, black bar) and s.d.
Among them, 16 (80%) showed gene editing at the target site of the IPA1 gene (Fig. 5g), which was much higher than the editing rate (27.5%) of IPA1 in the previous report (Li et al. 2016).
The editing rate of each nucleotide position was calculated.
Then the editing rate was transformed into standardized score (Z-score).
However, the editing rate at position 13 and 3'-end was similar between two libraries.
Although the editing rate was very high at position 19, there was no difference between two libraries.
Similar(47)
d, On-target editing rates for sgRNA variants plotted on a linear scale.
Supplementary Figure 7 Flow cytometry and amplicon sequencing produce similar editing rates.
c, Dose-dependent editing rates with Cas9 coupled with MS-sgRNA-1617 MS-sgRNA-1617 MS-sgRNA-1617enhander and -e2 targetargeting1A exon2 in CD34+ HSPCs BCL11Aenhanceris.
In induced pluripotent stem cells (iPSCs), we achieved precise genome editing rates of up to 45% by co-delivering the Cas9 RNP and donor DNA.
By delivering CRISPR/CRISPR-associated protein 9 (Cas9) ribonucleoprotein (RNP) complexes under conditions of rapid B cell expansion, we achieved site-specific gene disruption at multiple loci in primary human B cells (with editing rates of up to 94%).
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