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The in silico analysis showed that OsCYP-25 interacts with different proteins involved in cell growth, differentiation, ribosome biogenesis, RNA metabolism, RNA editing, gene expression, signal transduction or stress response.
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Due to the diverse impact of RNA editing on gene expression and function, it is possible that the misregulation of RNA editing may play a role in tumorigenesis by either inactivating tumour suppressor or activating genes that promote tumour progression.
This further implies that ADAR proteins affect editing and gene expression independently.
The programmable sequence specificity of Cas9 has been harnessed for genome editing and gene expression control in many organisms.
Next, we describe CRISPR/Cas-derived technologies for bacterial strain development, including genome editing and gene expression regulation applications.
In addition to their applications in genome editing and gene expression regulation, programmable DNA recognition systems, including both CRISPR and TALE, have been recently engineered for the visualization of endogenous genomic elements in living cells.
In addition, TALE nucleases have successfully modified human stem cells, allowing editing and gene expression tools for tissue engineering.
Despite the seemingly extensive crosstalk between RNA editing and gene expression, transcriptional regulation remains one aspect that is the least explored to which ADAR1 may make a contribution.
RNA-seq data provide information on editing and gene expression in the same samples, and thus allow us to assess the connection between the two.
There is much potential for editing and/or correcting gene expression in somatic cells toward a new era of functional genomics and personalized medicine.
Lack of an intact Cas9/intron-sgRNA gene in cells carrying either of two different edited genes strongly suggested that editing was due to transient expression of the Cas9/intron-sgRNA gene and the likely toxicity of long-term expression of Cas9 in C. reinhardtii cells.
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