Suggestions(3)
Exact(18)
Predicted editing ratios of nine A-to-I sites in editing box at chr2: 37,328,008 -37,328,100 are listed in all examined cell lines/tissues.
Although editing box sites were largely from Alu elements, 50 and 116 editing box sites were from non-Alu repetitive or non-repetitive regions, respectively.
Strikingly, clear differences of editing levels in the same editing box sites but from different tissues/cell-lines were also observed, strongly indicating a spatial and dynamic regulation of A-to-I RNA editing across human tissues.
Sequences of editing box region (Additional file 13) were cloned into the pEGFP series vectors [ 12] and each plasmid was transfected into HeLa cells for 24 hours prior to harvest total RNAs for editing analysis.
EB: Editing box; ESC: Embryonic stem cell; FPKM: Fragments per kilobase per million; gDNA: Genomic DNA; gSNP: Genomic SNP; HPB: Hits per billion-mapped-bases; RPKM: Reads per kilobase per million; PSS: Potential SNP score; SNP: Single nucleotide polymorphisms.
In an intergenic region between genes CCDC75 and EIF2AK2 in chromosome 2, two non-repetitive editing boxes (purple bars in Figure 5A) and one Alu editing box (one of IRAlus, pink bar in Figure 5A) are separated by over 1 kb.
Similar(42)
Thus, We named these regions containing promiscuous edited A-to-I sites as "editing boxes".
Finally, although functionally unknown, marked differences of editing ratios in the same sites identified in editing boxes clearly suggest a spatial and dynamic regulation of A-to-I RNA editing across human tissues.
Although we could identify no consensus sequences in non-repetitive editing boxes, they are likely to form dsRNAs and the edited sites have similar 5′ neighbor preferences as reported recently for other ADAR1 substrates [ 28].
They will change into editing boxes, like those to rename a file on your computer desktop.
The Streak team sees a number of use cases, including creating and editing boxes (useful for setting up web forms that submit straight to Streak); creating and editing pipelines; setting up automated reminders; searching through Streak data and getting a newsfeed for updates to a customer's boxes and pipelines.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com