Haemorrhagic areas, microcysts and necrosis as well as the fields at the edge of tissue sections were avoided.
For the purposes of this study, a positive margin was considered to be disease identified at ≤1 mm from the inked edge of tissue.
Notably, all thirteen bone cancers occurred before 25 years follow-up, nine of which developed inside or on the edge of tissue directly irradiated to treat the original bone sarcoma and one in a survivor diagnosed with a p53 mutation.
The recorded information on the distance between the tumor and outer edge of tissue sample removed during biopsy or CRC resection was used to categorize patients as having clear (no cancer cells at outer edge of sample), positive (cancer cells present at or close to the edge) or unknown surgical margins.
A concentration of 1-10 μg/mL of beta-actin dsRNA led to distinct observable morphologies; including loss of contact of the growing edge of tissue with the culture plate, loss of definition in the canal structure of the sponge, and poorly defined choanocyte chambers (Additional File 1, Figure S6A,B,C).
Contractile actomyosin purse-strings generally appear in the cleavage furrow of dividing cells (Schroeder, 1973; Yumura and Uyeda, 2003; Yumura et al., 1984), in the apical region of epithelial cells during embryonic morphogenesis (Sawyer et al., 2010), and at the edge of tissue wounds (Abreu-Blanco et al., 2011b).
In accordance with those findings, K8 positive cells with the highest expression levels often came to localize at the edge of tissue protruding into the submucosa, eventually generating a remarkable lining of K8high cells, i.e. cells displaying strong K8 expression.
But blood clots can also speed healing, because they "provide a bridge" between the torn edges of damaged tissue over which new tissue can grow, repairing the tear, Dr. Murray said.
Therefore, the outside edges of the tissue were not analyzed by XAS, whereas these tissue components were analyzed by biochemical assay.
