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The width of pericoronal radiolucency was measured electronically in millimeters as the greatest width from the middle of the mesial, distal, and occlusal planes either alone or simultaneously to the edge of the bone (Fig. 3).
Sequential double labeling also confirms that new bone addition forms the veil developing along the vj edge of the bone between 7 and 13 dpf (Figure 3E, arrowhead), the veils developing along the jp edge between 13 and 19 dpf (Figure 3F, asterisk), and the spur developing during this entire 12-day period at the j apex.
The metal tibia component was thus automatically marked and removed from the densitometry measurement and the edge of the bone was automatically outlined, but manually adjusted when needed.
A chamber was made by building a wall with dental cement (Paladur, Heraeus Kulzer, Hanau, Germany) along the edge of the bone covering the left hemisphere.
The next step is to automatically select seeds that have a high chance of being located close to the edge of the bone segments.
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The outer edge of the bones, were EFS would have been found, was well-preserved, and still lacked any fossilized proof of them being present for Ampelosaurus.
All the prepared implants were wrapped snug around the ends of the bone defect and the edges of the bone membrane sutured with a 7-0 atraumatic suture.
Histological analyses were made after staining with haematoxylin and eosin and Von Kossa, and immunostaining, and confirmed viable cells that stained for CD90, CD73, CD166, runt-related transcription factor, osteopontin, and collagen type I in the experimental group, while in the control group there was only connective tissue on the edges of the bone in the injury zone.
The edges of the bone in these lesions varies from tapered to sub-rounded, but all exhibit extensive surrounding remodelling of the bone surface texture, occasionally accompanied by mild rugosity of the bone surface, as is seen in FMNH PR2081 [3, Supporting Information S1].
Inflammation appears associated with osteoporosis of the trabecular bone, but also with new bone formation occurring at the edges of the bone, two contrasting features in close proximity.
This new bone starts forming from the osteotomy cuts towards the center forming a fibrous radiolucent interzone between the edges of the bone segments [ 20, 23, 27, 28].
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