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LCBs that contained a bias of single nucleotide polymorphism (SNP) density toward the edge of the alignment could have been the result of alignment artifacts.
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The latter two rules are designed to ensure that the outer edges of the alignment are covered even if the reference alignment contains partial sequences.
The alignment was then manually adjusted to ensure consistency with pfam00379, particularly at the edges of the alignment, and to ensure that alignments among sets of highly similar genes were not distorted by the global optimum.
The SBP-box genes used in this study are summarized in Additional file 2. We aligned full-length SBP-box protein sequences using Clustal X [ 26] and manually trimmed the edges of the alignment and discarded the excessive gaps.
In this step, we only allowed overlaps or gaps of up to 10 nt within the fusion junction to accommodate small errors in alignment that occur at the edges of the alignment.
In accommodating small errors in alignment that occur at the edges of the alignment, we only allowed overlaps or gaps of up to 10 nt within the fusion junction.
Otherwise, the alignments in A(C) are grouped by their pairwise overlap values, resulting in a partitioning of the positions in C based on the edges of the alignments in each group.
The alignment of a read-half determines an outside edge of the spliced read alignment, but does not determine where the exon-intron boundary occurs.
Clemens struggled to pitch into the fifth, but Pettitte and Mussina were not needed, and those innings that Clemens provided have given the Yankees what appears to be a monumental edge in the alignment of the pitching rotations for the American League Championship Series, which begins here today.
Use the edge of the toilet paper for alignment.
Since the number of vertices and edges in the alignment graph is a function of the genetic diversity of the sequences and not the sequence lengths, this method allows for a compact representation and fast alignment of very long and highly similar sequences.
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