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ECs (1 × 10 cells/ml) were added to each insert.
Subconfluent ECs (1 × 10) were harvested and washed twice with 10 ml of ice-cold PBS.
Then, the cells were washed three times with ECS and resuspended in ECS (1 × 10/mL).
To examine the migration of ECs, 1 × 10 cells in 100 μl of medium were added to the top of each transwell membrane.
ECs (1 × 10 cells per well) in 96-well plate were starved in basal CSC medium containing 2% FBS overnight before treated with 1 μM ISM and 15 ng/ml VEGF for 24 h.
For the determination of the proliferation rate of ECs, 1 μCi of [H]-thymidine was added to each of the wells prior to the final 6 hours of culturing, and the incorporated radioactivity was counted with a scintillation counter.
Similar(53)
To determine whether endothelial NO could modulate MΦ inflammatory activity, we cocultured thioglycollate-elicited peritoneal MΦ with ECs (1 1 ratio) in the presence of Fum-PD.
He warns the reverberations of losing "a little disco in EC1" will spread far beyond its walls.
After co-culture with SSc-ECs: 1) HC-MSCs showed a significant increase of α- SMA and collagen-related genes when compared to their basal levels (α- SMA, P = 0.0002; Col1A1, P = 0.0002; Col1A2, P = 0.0002); 2) SSc-MSCs did not show any difference in the expression of these genes when compared to their basal conditions.
oxidoreductase (EC 1), transferase (EC 2), hydrolase (EC 3), lyase (EC 4), isomerase (EC 5), ligase (EC 6).
In the metabolic network of C. salexigens, hydrolases (EC 3) were positioned primarily, followed by transferases (EC 2), ligases (EC 6), oxidoreductases (EC 1), lyases (EC 4), and isomerases (EC 5).
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