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Our findings illustrate rare co-occurrence of D. parvula and D. retrocurva in the field with strong spatial segregation in ecologically differentiated lakes supporting ecological isolation.
Under this evolutionary regime, ecologically differentiated individuals are not genetically differentiated at the vast majority of polymorphic loci.
Our second aim was to cluster short tag sequences into ecologically differentiated populations to shed light on the evolutionary ecological processes underlying microbial diversity patterns in the RT.
To test whether Cuvierina morphotypes were ecologically differentiated based on their biogeographic distributions, we applied ecological niche modelling (ENM) to estimate their ecological tolerances.
Finally, it is unlikely that interactions with the 200 g pale fork-marked lemur (Phaner pallescens) are shaping ecological structure of this community, as this species is ecologically differentiated from its family members due to feeding specialization on gum exudates and vertically separated by its habitat use [ 41, 44, 45].
Based on our morphological analysis, we predicted large differences between the ecologically differentiated forms in their functional performance when feeding.
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These potential positive effects of dispersal on speciation have been suggested in several laboratory studies reporting that mating isolation between ecologically-differentiated populations is stronger when they can exchange migrants in nature [11] [13].
However, these incipient daughter 'species' cannot stably coexist, because they are ecologically not differentiated but nevertheless have to compete for the same resources.
Most of these closely related species are morphologically and ecologically clearly differentiated (for examples see [ 13]), and several species are narrow endemics restricted to small areas.
In summary, although thick-lipped species are common in cichlid radiations, and A. citrinellus and A. labiatus are morphologically and ecologically clearly differentiated, they remain genetically surprisingly indistinguishable (Additional file 2, Figure S2).
The parental lines of our panel came from D. magna populations that are strongly differentiated ecologically, about 1500 kilometers apart.
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