Sentence examples for eco packaging from inspiring English sources

The retrovirus was generated by transfecting WSX1-IRES-GFP, WSX1-MUT-DSRed-IRES-GFP, or control GFP and DsRed constructs into Phoenix Eco packaging cells.

The Phoenix Eco packaging cells were transfected with pLNCX2-EGFP using calcium phosphate-based transfection.

High-titer, replication-in-competent retroviral particles were produced in Phoenix Eco packaging cell line (Orbigen, San Diego, CA).

High-titre, replication-incompetent retroviral particles encoding the RNA of interest were produced in the Phoenix Eco packaging line (Orbigen, San Diego, CA, USA) for murine melanocytes.

For stable transduction, retroviruses were produced in Phoenix Eco packaging cells, following the Nolan Lab protocol (http://www.stanford.edu/group/nolan/protocols/pro_helper_dep.html).edu/group/nolan/protocols/pro_helper_dep.html

Virus-producing RetroPack PT67 cells were established through a ping-pong method, which uses the virus from the Eco packaging cell line to infect RetroPack PT67 cells.

The pFB-hDicer construct was introduced into 293T cells together with the pCL-Eco packaging plasmid by calcium phosphate transfection.

Retroviral supernatants from of Phoenix-Eco packaging cells cultured at 37°C were collected after 48 h.

High-titre viruses were produced by transient transfection of retroviral constructs into the Phoenix-Eco packaging cell line using FuGENE HD transfection reagent (Roche) according to standard procedures.

For introduction of an activated Ras allele and Rb loss into astrocytes, Phoenix-Eco packaging cells (a gift from G.P. Nolan) were transfected with pBABE, pBABE-HRasV12, PIG-puro and PIG-CRE retroviral plasmids (a gift from P.P. Pandolfi).

Retroviral constructs (1 ug of pMSCV-ires-GFP to generate pMSCV-Pax3-ires-GFP and pMSCV-Pax7-ires-GFP [14]) were co-transfected with 1 ug pCL-Eco packaging constructs using Fugine 6 (6 ul, Roche).