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Volunteers refrained from eating 1 h prior to donation, and rinsed their mouths twice with 10 ml of bottled still water (Waitrose, Bracknell, UK).
Dental plaques were individually collected at least 2 h after eating and 6 h after tooth brushing, and DNA was extracted and then was disrupted into fragments with ~180 bp in length.
A total of 33 patients also received nicotinamide (80 mg kg−1) given orally at least 1 h after eating and 1.5 h prior to radiotherapy; 31 patients did not receive nicotinamide either due to coexisting vascular disease or patient refusal.
To avoid interfering with alcohol absorption, subjects were requested to avoid consuming alcohol for 24 h and refrain from eating for 6 h prior to the study appointment.
Subjects were asked not to eat 3 h before start of the experiment.
All were instructed not to eat 2 h prior to the experiment.
Subjects were given a light standard meal (∼200 kcal with 8 g fat) 30 min before each drug dose and were allowed to eat 4 h after administration of the study drug.
In 1989, Rehrer et al 8 found no association between the time of the last meal and GI disturbances, but almost all athletes ate 3 5 h before the race.
Participants presented for imaging in the afternoon at 1 00 p.m. or 2 30 p.m. with instructions to have eaten ~2 h prior to the scanning session so that they were neither intensely hungry nor full.
In a clinical trial carried out on 12 morbidly obese patients, atorvastatin was administered as an immediate release tablet of 20 80 mg in fasted state where patients were allowed to eat 2 h after administration.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com