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There are natural fears, not all unfounded, about a sudden tide of foreign people, and in the light of violent events around Europe it is easy to amplify them.
In our studies within Gentianaceae, trnH-psbA is often easy to amplify and sequence, even from degraded samples, and shows high levels of interspecific and intraspecific sequence variation.
As a result, ITS2 sequences are relatively easy to amplify using one pair of universal primers selected by our group (Table S1).
Then, by assembling the above exons with deliberately designed overlapping primers, it is easy to amplify the full-length cDNA or ORF of interest with software-optimized, highly efficient outmost primers.
There is therefore a trade-off between using genes that are easy to amplify in all species, but which by their nature are highly conserved and underestimate the true number of species, and using genes that give a better description of the number of species, but which are more difficult to amplify.
DNA is easy to amplify from pure cultures, but problems arise with contaminated samples, such as samples from biowaste.
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The greenhouse effect appears a lot easier to amplify than to reduce.
A simple truth of human existence is that it is vastly easier to amplify fear than it is to assuage it.
The extent of this problem was ascertained by comparing mitochondrial DNA sequences amplified from the Neanderthal libraries with those of modern humans; because mitochondrial genomes are so small, they are much easier to amplify, and prior work had already demonstrated that Neanderthal mitochondrial DNA sequences were distinct from those of modern humans.
We hypothesized that a chimaeric flocculation gene made up of a FLO1 variant derived from S. cerevisiae S288c and additional repeats from FLONF from S. cerevisiae CCUG 53310 would be more stable and easier to amplify by PCR.
A low threshold of 4.0 μJ/cm2 for ASE with a high quality factor (Q-factor) of 159 were achieved for POFP thin films, indicating that it is easier to amplify by excitation with an extremely narrow ASE compared with other polymer dyes.
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