This procedure is much less laborious and easier than fusion and recombinant expression of whole antibody-antigen fusion proteins.
It's an art form that defies easy description: a fusion of dance, music, poetry, classical dressage and shamanistic magic.
The LE-cloning strategy has been especially designed to allow the continuous and easy integration of fusion partners in different orders into a growing vector system.
It is worth emphasizing that the recombination systems of Cre/loxP and Gateway technology have proved to be very efficient and reliable for cloning, and allow easy manipulation of fusion constructs [19], [31].
In addition, with radiolucency, Fidji cervical cage does not produce artefacts on radiographs or CT scan, which makes it easy to evaluate fusion status.
Second, the DNA is more stable than RNA although it is easier to map fusion transcript if it exists, such as BCR-ABL.
When compared to early fusion, late fusion is easier to perform, but it cannot learn the correlation among features.
But the harsh truth is it's not at all easy to run this fusion process in a controlled way.
The design of the system allowed easy monitoring of soluble fusion protein expression by following GFP fluorescence, and rapid purification of the target protein through the intein-mediated cleavage reaction.
The easy joke is that fusion energy is the power source of the future and always will be, but what are its real prospects?
