Sentence examples for easy cloning from inspiring English sources

pELMO and pGEM-T Easy cloning efficiency regarding low, medium and large sized inserts.

For easy cloning of any antibody gene without potential modification of the antibody sequence, restriction sites were introduced in the pelB sequence and following the termination codon.

Plasmid identity was confirmed by sequencing, using the ccdBsec-F/ccdBsec-R and SP6/T7 primer sets; these were then aligned with the corresponding pELMO and pGEM-T Easy cloning site flanking sequences.

Every Gateway vector possesses att sites necessary for recombination and easy cloning of cDNA.

PCR products were cloned using the pGEM-T Easy cloning system (Clontech) and sequenced with Applied Biosystems Big dye terminator kit v1.1 and the ABCB7 forward primer.

The amplified fragment was cloned into pGEM-T easy cloning vector (Promega), sequenced.

These two primers were used in a PCR amplification with Pwo polymerase (Roche), using the pGEM-T Easy clone of the intron-containing promoter as template.

The amplicon was cloned into the pGEM™-Easy cloning vector (Promega, Mannheim, Germany).

Clone libraries were created using the pGemT-easy cloning kit (Promega) in accordance with the manufacturers instructions.

Each of these DNA fragments was inserted into pGEM T-easy cloning vector to obtain 10 or more independent clones for sequence analysis.

Amplification products were cloned in the pGEMT-Easy cloning vector (Promega Co, Madison, WI, USA) and the insert of at least 10 different colonies of each product were sequenced.