Sentence examples for easy buffer from inspiring English sources

All microarrays slides were prehybridized using 100 μL of DIG easy buffer (Roche Applied Science, Meylan, France) in humidified chambers for 1 hr at 42°C.

Blots were pre-hybridized in Dig Easy Hyb buffer and then probed with 100ng/ml Digitonin-labeled anti-sense RNA in Dig Easy Hyb buffer (Roche) at 65°C overnight.

Telomere length was determined by probing with a digoxigenin (DIG) end-labeled telomere probe (TTAGGG 3 at 42 C for 12 hours in DIG Easy Hyb buffer (Roche, Indianapolis, IN).

Prehybridization and hybridization were carried out at 41°C overnight in DIG Easy Hyb buffer solution (Roche).

DNA gel blots were hybridized at 42°C in a DIG easy hybridization buffer (Roche Co., Germany).

The blots were prehybridized at 42°C for at least two hours in Dig Easy Hyb buffer (Roche Corporation, Indianapolis, IN).

Digoxigenin-labeled probes to aaap were produced using the PCR DIG Probe Synthesis Kit (Roche Corporation) and hybridized overnight at 42°C in DIG Easy Hyb buffer.

Prehybridization was performed with 100 μl DIG Easy Hyb buffer (Roche, Indianapolis, IN) for all microarray slides in a humidified chamber for 1 h at 42 °C.

The membrane was hybridized over night in DIG Easy Hyb buffer (Roche, cat#11603558001) with DIG labeled probe generated by PCR DIG probe Synthesis kit (Roche, cat#11636090910).

DIG-labeled probe was hybridized in DIG Easy Hyb buffer (Roche) overnight at 50°C and washed at moderate stringency according to the manufacturer's instructions.

The second-stage PCR reactions included the following in a total volume of 50 µl: 1 µl each of the first-stage 5′ and 3′ flanking sequence PCR products, 100 ng pFA6-kanMX6 (Bahler et al. 1998), 5 µl 10× Easy A buffer, 4 µl dNTPs (stock is 2.5 mM each), and 0.5 µl (2.5 U) Easy A Hi-Fi polymerase (Agilent Technologies, Santa Clara, CA).