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Mariner elements seem to escape easily transposition controls since huge copy number are observed for several lineages.
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Finally, some aspects of the data cannot be easily attributed to demography or transposition and thus may reflect differences due to host mating system.
The dual-component system, which consists of an integrative plasmid with a mini-Mu and an easily eliminated helper plasmid encoding inducible transposition factors, is described in detail as a tool for the integration/amplification of recombinant DNAs.
Visible markers, such as white +, may be engineered to be a part of the transposon, and multi-transposition events can be easily selected out by the presence or absence of markers at the early stages of screening.
Despite the fact that the tri-cistronic transposon vector is fairly long (8.3-kb) and, moreover, that HaCaT cells are difficult to transfect, robust transposition of the genetic sensor was easily achieved allowing production of stably transfected cell lines.
Results from a typical Basic Local Alignment Search Tool (BLAST) analyses do not easily indicate whether there is a gene loss or transposition.
To investigate if the transposition of mGing or mPing was more easily detected in regenerated plantlets, two experimental systems were designed to examine the transposition frequency of mGing/ mPing in calli.
Lateral, central, and medial fat pockets are easily teased from their respective compartments using blunt forceps for transposition over the orbital rim (Fig. 4).
One form of transposition (permutation) that was widely used depends on an easily remembered key word for identifying the route in which the columns of a rectangular matrix are to be read.
To me, it was a simple transposition of yes for no, or up for down, and easily corrected.
In manual systems transpositions are generally carried out with the aid of an easily remembered mnemonic.
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