Sentence examples for ears were fixed for from inspiring English sources
Freshly dissected inner ears were fixed for 3 to 4 hours in 2.5% gluteraldehyde in 0.1 M phosphate buffer pH 7.3 at 4°C.
For Cdc42 antibody staining, dissected inner ears were fixed in ice-cold 10% trichloroacetic acid (TCA) for 1 h.
For SEM, inner ears were fixed in 2.5% glutaraldehyde in cacodylate buffer and then treated using standard procedures.
The end organs of ears were fixed in 2.5% glutaraldehyde overnight followed by several washes with 0.1 M phosphate buffer and then fixed with 1% osmium tetroxide for up to 1 hour.
The dissected ears were fixed in 0.4% paraformaldehyde, dehydrated in 100% methanol and rehydrated and then digested briefly with 20 µg/ml of Proteinase K (Ambion, Austin, TX, USA) for 15 20 minutes.
Briefly, whole-inner ears were fixed briefly in ice-cold 4% paraformaldehyde to help maintain structural stability and processed for cryosectioning.
We chose breeds with either drop or non-drop ears that were fixed for the appropriate alleles at associated SNPs in the GWAS analyses and the segregation of associated markers presented in ref. [ 21].
After removal of the temporal bone, the inner ear was fixed in 4% PFA overnight at 4°C and decalcified in 10% EDTA for 3 days at 4°C.
