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P6 mice were perfused with 4% PFA in PBS, and the inner ears were dissected out and post-fixed in 4% PFA in PBS for 2 hr at 4°C.
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The inner ears of P8 Ildr1 w+/+ and Ildr1 w−/− littermates were removed from animals after decapitation, and the cochleae were dissected out in Leibowitz media (L-15, Invitrogen).
Striatum were dissected out and stored at −80°C until analysis.
Neural tubes were dissected out as described earlier33.
Fetal organs were dissected out, washed three times and mechanically dissociated to small pieces.
At the end of the experiment, the tissues were dissected out for histopathological evaluation.
The L4-L5 SC were dissected out and post-fixed in 4% paraformaldehyde overnight at 4 °C.
After 7 days, TGs were dissected out and immunostained with an anti-TRPV1 antibody.
The brains were dissected out and post-fixed in the same fixative for 12 h.
Spleens were dissected out and weighed.
Organs were dissected out and the wet weights were determined.
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CEO of Professional Science Editing for Scientists @ prosciediting.com