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We find that hub regions and a backbone of pathways facilitate early spreading, while the shortest path structure of the connectome enables cooperative effects, accelerating the spread of cascades.
We therefore treated early spreading myoblast cells with the Src inhibitor PP2 to observe the effect on podosome assembly.
The time course of the early spreading events in representative cells (bone-specific SCP2, lung-specific SCP3, non-metastatic SCP21, and control MCF10A cells) was quantified (Figure 6).
This result suggests that the action of MLCK and Rho-kinase may be spatially segregated in early spreading as they are in fully spread cells [42].
We previously observed that isotropic cell spreading can be divided into three phases based on the rate of contact area increase: early spreading (P0), middle spreading (P1), and late spreading (P2) [25].
Cell spreading is a commonly used model system for motility experiments – spreading fibroblasts exhibit stereotypic, spatially-isotropic edge dynamics during a reproducible sequence of functional phases: 1) During early spreading, cells form initial contacts with the surface.
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They arrived early, spread a blanket on the grass, and waved at John, who was sitting at a picnic table, writing in his journal.
Early spread of the pandemic virus in both states was concentrated in the capital cities [3].
The Amerasian basin in the western Arctic Ocean had formed during an earlier spreading phase from about 130 to 110 million years ago.
By exciting only fluorophores within 100 nm of the substrate [31], TIRF revealed membrane dynamics at the earliest spreading times that the cell body usually occludes in bright field imaging (Fig. 1C).
We brought her here to Los Angeles, from her home in New York City after the lung cancer she was diagnosed with six months earlier spread to her liver.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com