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This is consistent with a common role for GLD-1 in regulating gene expression in germline mitotic cells, early-stage oocytes, and the early nematode embryo (Jones et al. 1996; Nayak et al. 2005).
Their conservation among multiple nematode species, especially of those spanning all the four nematode clades (Table 2) (phylogeny based on [ 38]) included in this study (see below), suggests that they may have emerged in early nematode ancestors after they diverged from other animals, and they may include the molecular determinants archetypical to the phylum Nematoda.
Because a hh gene has recently been identified in cnidarians, the most parsimonious explanation for these nematode specific gene families is that they are evolutionarily derived from a hh gene in early nematode evolution [ 12], and that the loss of the Hog domain in many of the genes is a secondary event, as earlier postulated [ 11].
The N-terminal domains of the C. elegans hh-related genes were not found in vertebrates and flies using blast searches, giving rise to the notion that these genes were perhaps derived from hh in early nematode evolution [ 17, 18].
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The C. elegans IFE-1, -2 and -5 appear to result from gene duplications that occurred within early nematodes to give rise to a specialized sub-class that recognizes alternate cap structures.
We found good agreement across optimality criteria with both Bayesian and Maximum Likelihood methods; our analyses revealed that the Dorylaimia and Enoplia split early from other nematode lineages, although tree topologies did not definitively resolve the branching order of these two groups.
Hutton and B. Morgan through earlier nematode tolerance assessments.
Thus, the initial duplications and neo-functionalization occurred early in nematode evolution, but have been followed by further change in separate Rhabditoid lineages, as different species experienced slightly different selection pressures for entry and persistence in their dauer forms.
Their results suggest the presence of strong selection early in nematode domestication for optimal growth under rich nutrient conditions similar to the significant GO term, "behavioral response to nutrients", found among lab-specific SNPs in fruit flies (Table 1).
Thus whilst evidence for rapid diversifying selection exists in the evolution of the hsp-90 gene (as evidenced by the significance of the branch-site tests) and most likely occurred early in nematode divergence, we have been unable to prove with confidence that the selection seen correlates with a change in the GA-binding function of Hsp-90.
Earlier frameworks identified both the Enoplia and Dorylaimia as early-branching nematode taxa [ 7, 22], although these studies reported a polytomy at the basal node.
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