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To test phagocytosis, early third stage larvae were injected with 69 nl of pHrodo Red E. coli BioParticles (1 mg/ml; Molecular Probes).
Parasitological examination at slaughter showed that Teladorsagia spp. and Haemonchus contortus survived the winter, both in the abomasal mucosa as adults and as early fourth-stage larvae (EL4).
For Teladorsagia spp., adult worms dominated in the abomasum, while for H. contortus early fourth-stage larvae (EL4) dominated (Table 1).
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Clones were induced in the early third instar stage and recovered in the late third instar stage.
Clones induced in the early third instar stage and recovered in the late third instar stage again consisted of two relatively large, differentially labeled daughter cell clones that were similar in size and cell number indicating a symmetric division mode.
ASP development depends on Wg expression at the early third instar stage.
In contrast, from early third instar stage onward, the axis of cell division shows an oblique to orthogonal orientation relative to the disc surface.
The expression of these Halloween genes is initially quite low during the early third-instar stage and then rises, as previously reported (McBrayer et al., 2007).
It does not survive the Nordic winter on pasture, but with almost 100%% arrested development in the early fourth larval stage it is capable of surviving the Nordic winter within its host [ 3].
Despite the fact that the parasite populations were at least 10 weeks of age, 73% of the H. contortus and 55% of the T. circumcincta burdens in the Control lambs were in the early fourth larval stage (early L4) of development.
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