Thus, functional peculiarities of early embryos allow studying the nuclei under different transcriptional conditions without artificial inhibitors.
Finally, ubiquitous expression of a UASp- Pex1 transgene via Tub-GAL4 in the early embryo allowed homozygous l(3)70Da s4868 and l(3)70Da mutants to survive past the second larval instar, confirming that the early larval lethality observed in these mutants was caused by loss of Pex1.
Early Drosophila embryos allow an efficient isolation of native mitotic chromosomes and thereby imaging with reduced background.
To induce anterior neuroectoderm, we injected chd mRNA, which encodes a potent BMP antagonist, into early embryos and allowed the explants to develop until stage 15 (Sasai et al., 1995).
The translucent nature of zebrafish embryos allows a mechanistic understanding of the origins of these defects to be established by tracking organ development, starting as early as 1 dpf.
First, external fertilization and transparency of zebrafish embryos allow better visualization of early blood-related phenotypes than mice, where development occurs in utero.
Because treatment of zebrafish embryos with Y27632 from early stages of development is lethal (33), we chose to treat embryos from 24 48 h post-fertilization (hpf) using a diluted solution of 100 n m, following which the drug was washed-out and the embryos allowed to develop until 4 days post-fertilization.
Zebrafish uniquely combine embryological manipulability, optical clarity of the early embryo and larvae (allowing simple visualization of cell biological events directly in vivo) and the ability to apply invertebrate-style forward genetics to questions of vertebrate development.
The down-regulation of E-Cadherin is key for 'epithelial to mesenchymal transitions' in the early embryo: these transitions allow future mesoderm cells to detach from their pluripotent neighbours and move to form the mesoderm (Nieto, 2013).
We showed that in silico and Affymetrix microarray approaches were relevant and complementary in order to find new avian genes potentially involved in oocyte maturation and/or early embryo development, and allowed the discovery of new potential chicken mature oocyte and chicken granulosa cell markers for future studies.
