Sentence examples for early confluence from inspiring English sources
For the scratch assay, the cells were seeded into 6-well plates at a density of 1 × 105 cells/well and cultured in a CO2 incubator until they reached early confluence.
When fibroblasts reached early confluence, fresh media containing TGF-ß TGF-ßyme, FraminGenzymeA) were added to the cultures.
At early confluence (24 hrs), the total number of cells was similar in KRIT1−/− and Lv-KRIT1 MEF cultures (Fig. 7E), suggesting that KRIT1 loss does not affect the rate of cell proliferation.
Fibroblasts (105 cells/well) were seeded in 12-well plates, and at early confluence, transiently cotransfected with reporter constructs and expression vectors or appropriate empty vectors using Superfect Reagent (Qiagen, Valencia, CA), or infected with adenoviruses.
Cells (ACCS, ACCY, TYS, HSG, and SG) grown in monolayers were harvested at early confluence.
Cells were studied between passages 4 and 8 at early confluence.
At ≤80% confluence, early passage HUVEC doesn't express EGFR, and therefore, the fusion receptor can be used to distinguish the signaling pathway that is mediated by NRP-1 [16].
Compared to static culture, bioreactor-cultured hfMSC scaffolds reached cellular confluence earlier (day 7 vs. day 28), with greater cellularity (2×, p < 0.01), and maintained high cellular viability in the core, which was 2000 μm from the surface.
In addition, Zhang et al. [ 71] recognized that scaffolds seeded by human fetal mesenchymal stem cell (hfMSC) reached cellular confluence earlier with greater cellularity and also conserved high cellular viability in the core of them compared to a static culture.
Increased protection against asthma therefore stems from a confluence of early-life microbial exposure, normal immune maturation, and low exposure to airway irritants or inflammatory factors.
Its name is the result of the fascinating confluence of an early government attempt at stemming the consumption of gin through taxation, and what might be construed as the world's first drive-up window.
