The highest stimulatory effect with maximum plant growth in terms of early sprouting, plant height, leaf area, chlorophyll contents, fresh and dry weights of tubers was observed in plants treated with silt+leaf compost+perlite combination; this combination significantly enhanced plant growth (30−150%) as compared to control.
The targeted nature of FIB/SEM was critical in relocating the area of interest and mapping the microanatomy of the early sprouts through volumes of ∼50,000 µm3.
As expected given the establishment of apical dominance at early sprouting [ 34], most, if not all, growing sprouts on control line K tubers were found at-or around-the apex, on the upper (apical) half of the tuber (Fig. 2c).
In different plant species early sprouting depends upon the air, water content and temperature of the medium (Guerin et al. 2001).
The growth of lymphatic vessels during early lymphatic development largely involves the sprouting of lymphatic endothelial cells from the veins (Srinivasan et al., 2007; Yaniv et al., 2006) and requires vascular endothelial growth factor 3 (VEGFR-3) signalling (Irrthum et al., 2000; Karkkainen et al., 2000, 2001; Veikkola et al., 2001).
We observed a significant increase in the sprouting of hepatic SECs into early stage, micrometastases.
Ligand-dependent activation of EPHA inhibits HGF-induced sprouting of cellular protrusions, an early step in the branching morphogenesis of Madin-Darby canine kidney (MDCK) cells [71].
The loss of sensory neurons following early nerve damage triggers axonal sprouting within the damaged nerve itself and collateral sprouting of adjacent intact afferent terminals into the denervated region of the dorsal horn.
Mouse and zebrafish models of lymphatic development are instrumental for gene discovery and gene characterization but are challenging for certain aspects, e.g. no direct accessibility of embryonic stages, or non-straightforward visualization of early lymphatic sprouting, respectively.
These data suggest, in practice, the feasibility of preventing early tuber sprouting during long-term storage by the ectopic expression of recombinant cystatins at low levels, with a minimal demand in endogenous amino acid resources and a likely negligible effect on the tuber protein complement.
