Earlier transcripts were then checked for the new concepts.
Earlier transcripts were then reviewed to check for data that could fall under these new codes.
Thematic analysis took an iterative approach, whereby as new themes were identified and added to the thematic framework, earlier transcripts were recoded [ 20].
An iterative procedure is followed in which new themes emerging from interviews are added to the coding frame and earlier transcripts recoded.
Data from earlier transcripts were analyzed concurrently with ongoing data collection [ 10, 12] in order to ensure that emerging themes could be further pursued in later interviews.
We created an initial set of codes collectively; as data review progressed, we refined and added codes, re-coding earlier transcripts to reflect changes.
After all transcripts were coded, every transcript was analyzed for a second time to ensure that all newly derived codes that came up in later transcripts were also checked in earlier transcripts.
Thematic analysis involved a constant comparison process, aligning new data with existing codes, generating new codes, reviewing earlier transcripts for new codes, grouping data into major and subthemes, and identifying negative cases.
Similar to results obtained with leaves analyzed 5 weeks earlier, transcripts of TRV1 but not TRV2 transcripts were detected, though RT-PCR analysis, in the 5 DAA fruit of the infiltrated plant 1 and neither of the viral transcripts was detected in the 5 DAA fruit of the not infiltrated, wild type plant, as it is shown in Figure 4C.
