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Following an earlier reported method the AAV2 plasmid pTRUF11 expressing fluorescent green protein gene under control of a hybrid promoter (cytomegalovirus enhancer and chicken β-actin) and simian virus 40 polyadenylation site was packaged into AAV5 [43].
The DNA protection was studied using supercoiled pUC18 plasmid DNA according to an earlier reported method [ 26], with minor modifications.
Singlet oxygen (O2) production, and at the same time, its scavenging by the samples and the reference compound lipoic acid can be monitored by N, N-dimethyl-4-nitrosoaniline (RNO) bleaching, using a earlier reported method [ 12].
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The present study is more versatile than the earlier reported methods.
It was observed that the proposed method produced detection rate that was comparable to earlier reported methods and recorded reduced time delays, at an average of 100 ms.
0.2 μg per sample of genomic DNA was used to carry out the PCR using specific primers in a 50 μl final volume; genotyping was carried out according to earlier reported methods for TYMS 5′UTR VNTR and G>C substitution (Gusella et al, 2006a), TYMS 3′UTR 6 bp insert-deletion (Ulrich et al, 2000), MTHFR C677T and MTHFR A1298C polymorphisms (Miao et al, 2002).
The optimised method and an early reported method for the gliadin separation were validated by evaluating linearity, sensitivity, detection and quantitation limits, repeatability and precision.
In this paper we report the extension of an earlier reported DNS method (Deen et al., 2012; Deen and Kuipers, 2013) based on a novel Immersed Boundary Method (IBM) which incorporates the fluid solid coupling at the level of the discrete field equations.
Early mobilization is the most commonly reported method of managing the healing flexor tendon.
In this study the enantioselective resolution was confirmed by estimating the enantiomeric purity of the product by using an earlier reported chiral HPLC method [33].
The samples prepared after extraction were analyzed by an earlier reported chiral HPLC method for the enantioselective resolution of racemic Carvedilol [33].
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