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The gate to the clinic where they had earlier prepared a tent for cholera treatment was locked because of a misunderstanding with Health Ministry officials, who thought the group planned to open on Monday, not Sunday.
Twenty milliliters of the standard control, or the unknown serum sample were pipetted into the appropriate wells followed by the addition of the primary antibody (150 μL), which was earlier prepared by mixing the primary antibody and primary incubation buffer in a ratio of 1 100.
The assessments were done according to the questionnaire with 26 items on the earlier prepared questionnaire on paper per park (see Table 1).
After completing all the calibration steps and finding that they were all carried out properly, the measurement of the earlier prepared sample was started.
Then the earlier prepared dispersion was emulsified in PVA solution.
The level of ALCAM transcripts from the earlier prepared cDNA was determined using a real-time quantitative PCR, based on the Amplifluor™ technology [ 26], modified from a method previously reported [ 22, 27].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com