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At 48 h post-challenge, ear swelling was measured using a spring-loaded micrometer (Mitutoyo) and the difference in ear thickness was calculated for each animal.
The inflammatory reaction was correlated with increased values in ear thickness.
After measuring ear thickness for each group, values for oedema and inhibitory rate were calculated (Figure 6 a, b).
Treatment with genistein and genistein incorporated in the two cyclodextrins led to decreased values for ear thickness.
In case of treatment with pure genistein, the average value for ear thickness decreased to 0.40 ± 0.03 mm.
Indomethacin was used as control and the average value of ear thickness in this case was 0.29 ± 0.04 mm.
The FITC-specific ear swelling was defined as follows: (right ear thickness at 24 h after elicitation - right ear thickness before elicitation - left elicitation - left4 h aftearelicithicknessft eat thickness before elicitation).
At 6 and 72 h after challenge ear thickness was measured using an engineer's micrometer, and compared with ear thickness prior to challenge.
Ear swelling was calculated as the difference of swelling between the right and the left ear (ear swelling = ear thickness after challenge – ear thickness before challenge).
At 6 and 72 h after challenge, ear thickness in both Jak3+/+ and Jak3−/− mice was also measured and compared with ear thickness unchallenged.
Swelling, as major read-out, was followed after challenge by measuring ear thickness.
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