Exact(1)
This much earlier evidence for a fully functional ear challenges a leading hypothesis for why ears evolved in the first place.
Similar(59)
In effect, it threatens to turn the whole taxi system on its ear, challenging the logic of the way the medallion industry is regulated.
Subsequently, recipients were ear challenged and the ear swelling was assessed.
Mice that were ear challenged without prior sensitization served as negative controls.
Mice were ear challenged 5 days later with 0,14% DNFB and the CHS response was determined as described above.
On day 5, mice were ear challenged with 0.15% DNFB applied onto the right ear; the left ear received the vehicle alone.
In some experiments 5×106 PBS- or α-MSH treated CD4+ or CD8+ T cells were injected intravenously into DNFB-sensitized mice 24 h prior to ear challenge.
Irrespectively of L. casei treatment, CD8+ T cells were hardly detectable in the skin of NaCl-treated control unsensitized mice at 48 h post ear challenge (Fig 3A and B, left panels).
Ear challenge of sensitized mice with the hapten resulted in the rapid recruitment of CD8+ T cells including both granzyme B+ and granzyme B− CD8+ CTL, which appeared within 24 hr after challenge and persisted for up to 72 hr (Fig 3A upper right panel, and Fig 3B left).
To investigate whether binding of α-MSH to MC-1R was required for the reduction of contact allergy CD8+ T cells from C57BL/6Je/e mice were stimulated with α-MSH, co-cultured with DNBS-pulsed DC, injected intravenously into DNFB-sensitized wt mice and subsequently, recipients were ear challenged to the hapten.
MACS-sorted CD4+CD25+ T cells harvested from naive C57Bl/6 donor mice treated by daily oral administration of L. casei or NaCl as control for 14 days, were co-transferred with CD8+ T cells from naive C57Bl/6 into syngenic CD3ε°/° recipients one day before skin sensitization and the CHS response to DNFB was examined after ear challenge with DNFB (Fig 6A).
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