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The seeds of 4 varieties (200 g each) were kept on the paddy from November 2009 to April 2010.
The medium-sized tortoises, weighing approximately 70 100 kg each, were kept together in an enclosure, where they were also being fed vegetables and other fruit throughout the feeding experiment.
The urine aliquots (1 ml each) were kept at -20°C.
The three study groups of 30 A. citrinellus individuals each were kept under standardized laboratory conditions with 12 h daylight for a period of six month.
For VIGS experiments, 10×10×10 cm pots with 4 germlings each were kept in a growth cabinet with a 16 h light period at 26°C and an 8 h dark period at 23°C.
Briefly, six (6) nulliparous and non-pregnant female Balb/c mice, about 10 weeks old (averagely 20 g each) were kept in their cages for 5 days prior to dosing to allow for acclimatization to the animal house conditions.
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One correct transformant each was kept for further studies.
A light/dark cycle of 12 h each was kept throughout the study periods.
Excitation and emission slit widths each was kept at 5 nm.
Samples from each tree and each tissue were kept separate for RNA extraction and gene expression studies.
Ten single-housed rats of each age were kept as water controls, and 10 group-housed rats of each age were kept as housing controls.
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