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All specific cohesin-dockerin interactions were of similar intensity, indicating that similar amounts of protein were bound in each well, supporting a cohesin:dockerin molar equivalent of 1 1.
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To estimate the probabilities of the possible ancestral states at each well supported node of the concatenated Bayesian topology we chose the Bayesian approach of posterior mapping [ 61, 62], using the program SIMMAP 1.0 [ 101] freely available online http://www.SIMMAP.com.
Within this group, we identify a total of seven primary lineages (Lineages 4 10), each well supported and geographically unified; and we note that the same seven lineages were recovered by Rowe et al. [ 16].
The remaining macroglossines are grouped into two clades, each moderately well supported; these are placed as sister groups in our tree, to the exclusion of Hemarina, but with very weak support.
Vertebrate alpha and beta chains constitute monophyletic groups and are each others well supported sisters.
Our study includes representatives of the Clinini and Myxodini and the monophyly of each is well supported.
Consistent with the previous results, all the 10 Lysinibacillus strains and Bacillus spp. NRRL B-14905 were grouped into one cluster, and the toxic L. sphaericus strains and L. fusiformis and L. boronitolerans each formed well supported subclusters.
Phylogenies within these clusters are generally congruent to each other and well supported at most nodes, but disagree with the well-supported Bac protein phylogeny.
Each lineage was well supported by >75% Bayesian posterior probability, p values≤0.001 in the zero branch length test, and >70% bootstrap.
The now established phylogenetic relationship that designates main Symbiodinium clades and types within each clade is well supported by a number of different genetic markers [ 13- 16].
Monophyly of the family and of each subfamily is well supported by our analyses of cpDNA across all monocots (Additional file 3: Figures S2 and S3) and by other recent studies [ 28- 30].
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