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Each well was measured once.
Each well was then triturated and the supernatants were centrifuged.
The supernatant of each well was collected for cytokine analysis.
The final volume in each well was 200 μL.
The distance between each well was 100 m.
Each well was added by another filter paper disk.
The medium of each well was changed twice per week.
Production from each well is also tracked every time step.
Colour change in each well was monitored visually.
Subsequently, the supernatant in each well was removed by pipetting.
The solution of each well were transferred to a 96-well plate.
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