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Genomic DNA (2 µg each) was digested to completion by overnight incubation with Mse I, Bfa I, and Csp 6I (New England Biolabs, Ipswich, MA), respectively (See Figure 1B left panel).

For each breed, a total of 25 µg from each DNA pool, divided in five aliquots of 5 µg each, was digested with each of three restriction enzymes AluI, HaeIII and MspI (Fermentas GmbH, St. Leon-Rot, Germany), following the manufacturer's recommendations.

To find the cDNA of a representative sequence (tag) from the SAGE libraries, double-stranded cDNA of the whitefish and omul brain (of the same two individuals that were used for SAGE, 3  μg each) was digested overnight with PvuII restriction enzyme (100 U, Fermentas) in a volume of 300  μL.

In brief, two aliquots of 250 ng of DNA each are digested with NspI and StyI, respectively, an adaptor is ligated and molecules are then fragmented and labeled.

Genomic DNAs isolated from P. westermani adult worms (5 μg per each) were digested with restriction enzymes, Acs I, Sac I and Sfu I.

Amplified samples and references (1 μg each) were digested with DNaseI and labelled with Cy-5 dUTP and Cy-3 dUTP, respectively, using the BioPrime labelling kit (Life Technologies).

Genomic DNA from each strain was digested, electrophoretically separated, and Southern blot (Southern 2006) hybridization patterns for each selected element were then compared.

Approximately 1 cm long of tail from each mice was digested with Proteinase K butter.

After reconstitution in 50 mM triethylammonium bicarbonate (TEAB), 0.1% ProteaseMax, each sample was digested with trypsin (1 50) overnight at 37 °C.

A total of 50 µg protein from each sample was digested with trypsin for targeted proteomic analysis.

One nanogram of DNA from each pool was digested and fragments of insert size ranging from 500 to 550 bp were selected.