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Initially, each virus primarily affected young adults -- gay men for AIDS and health workers of both sexes for SARS.
"Each virus has a unique genetic signature and when that's known we know where the hell the bug came from.
Primers and/or probes of the two assays were designed from conserved regions of each virus.
Amplicons from each virus were cloned and sequenced to verify their identity.
The specificity of the GeXP assay was examined using positive controls for each virus.
Primers and probes were designed from conserved sequence stretches to detect all isolates of each virus.
Firstly the uniplex RT-PCR assay was standardized for the detection of each virus independently.
Specific primers and probes (Table 1) for each virus were added to the PCR assay along with DEPC water.
For each virus group, 19 to 23 mice were used.
The experiments were performed once or twice for each virus.
The database included the province for each virus isolate.
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