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We quantified cell viability in each well under the respective culture conditions in a resazurin cell viability assay and normalized each viability score to the mean of the non-silencing control siRNA on the same plate.
Each viability experiment included 5 6 wells per dose.
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As a first step, we expressed both constructs in a variety of tissues using different GAL4 driver lines and examined the effect of each on viability of the organism.
For each ampoule, viability was assessed on small aliquots of cells by propidium iodine staining; also the extend of DNA fragmentation was assessed with Comet essay in every replicate.
For each batch, viability tests have been performed for both starting materials and final product.
The culture medium was changed every 24 h through 96 h, and each time viability, proliferation, and morphology were assessed.
For each experiment, viability counts (log CFU/mL) were plotted against time and expressed as the means of results from three separate assays.
We then deleted each reaction and determined viability on each of the 10 carbon sources.
Suppose that a human population is host to these mobile elements, which reduce each individual's viability by one percent for each mobile element.
One coverslip from each embryo was stained for viability at each time point, yielding four coverslips for each genotype at each time point (DIV1, 4, 7, 14, 18).
HEK were treated with serial dilutions of each NM for 24 h and assessed with each of the viability assays.
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