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Three project phases are already completed: The parameter determination to indicate potential impact of chemicals on biological systems, the selection of test substances, as well as the manufacturing of 3 functional modules, each verified for use in ecotoxicological research.
For each verified binding site in our test set, we randomly select 20 genomic locations (from the same promoter sequence) with the average binding site of length 12, which results in a background set that is 20 times larger than the test set.
Significantly, a number of glycans were shown to specifically bind both isolates in a manner dependent on growth/maintenance conditions, including Man, Fuc, Gal and Neu5Ac, with each verified using a cell-based adherence assays (summarised in Table 3).
Specifically, for each verified mature miRNA, we used a same-size sliding window and selected all possible sequences which could be created by sliding 1 base pair towards either direction from the verified mature miRNA over the precursor sequence, excluding any hairpin loops.
The gene sequences in these plasmids were each verified by DNA sequence determination.
SJ coded the transcripts and CvE and LH each verified one-third of the transcripts as an inter-rater check.
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The registrations were each visually verified.
Information on each gene verified as differentially expressed is shown.
Dissociation curves run for each reaction verified the presence of a single amplicon peak.
Single PCR bands for each exon verified the specificity of the amplification prior to sequencing.
Interviews were transcribed verbatim, with the accuracy of each recording verified by the interviewer.
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CEO of Professional Science Editing for Scientists @ prosciediting.com