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The frequency of each variability class on specific positions was mapped on the homology model of PsbO (Fig. 4).
We compared the helix-forming propensities of natural mRNAs with those of the mutants, vis-à-vis mRNA length, for all sites as well as separately for each variability category of sites for the two species, Psilotum nudum and Nephroselmis olivacea.
The outcome of the variability analysis was summarized over two intervals (30 minutes immediately prior to SBT start and the first 30 minutes of the SBT), by computing the median of each variability time series within these intervals (excluding windows that contained the SBT start time).
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Thus, the contribution of each variable to the variability in CTTOT could be inferred.
LHS provides a good coverage of each parameter variability by dividing each parameter range into n (sample size) equal-probability subintervals, which are sampled exactly once.
The Y-coordinate of each ellipse (variability ranking value) is then determined by the surface area A of each ellipse, which is proportional to phenotype variability as defined in the PCA space.
Within each mouse, variability between slides or between fields within slides had smaller effects on the error of the analyses.
For each species, variability in Hg concentrations reported in the literature was summarized using the mean ± SD and median of the observed data.
To assess intra-reader variability, each scan was evaluated 10 times by each reader.
Within each cohort, interassay variability was ensured by including identical reference samples on each assay plate, and inter-plate variability controlled by correcting for these references.
For assessment of interrater variability, each of two blinded investigators scored the flow in each sample independently.
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