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12 Doctors we saw, including having three magnetic resonance imaging scans each, two times getting blood drawn, two times being exposed from the waist up, 15 minutes on a treadmill for a VO2 max test, one X-ray and one gynecological (no, you did not read that incorrectly) checkup.
We recorded 432 utterances (216 words, repeating each two times) included in the ATR Japanese speech database [28].
The slides were washed two times in 2× SSCT-50% formamide for 30 min each, two times in 2× SSCT and two times in 0.2× SSCT for 30 min each.
Membranes were then incubated with secondary antibodies at 37°C for 2 h, washed four times with TBS/Tween 20 for 15 min each, two times with TBS for 5 min each and detected using a DAB HRP (horseradish peroxidase) colour development kit (Beyotime).
After two rinses (10 minutes each) with 1% NDS in wash solution, sections were incubated for 2 hours at RT with the appropriate secondary antibodies conjugated with Alexa dyes (Invitrogen, Carlsbad, CA, USA), then washed (10 minutes each) two times with 1% NDS in wash solution and two more times with PBS, and mounted on slides.
The membrane was washed two times with 2 × SSC and 0.05% SDS at room temperature (RT) for 5 min each, two times with 0.1 × SSC and 0.1% SDS at 50 °C for 15 min each and one time with MABT (0.1 M maleic acid, 0.15 M NaCl, 0.3% Tween-20, pH 7.5) at RT for 5 min.
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Cerebral palsy victims now get £6m each – three times the amounts courts awarded a decade ago.
Do each three times in a row with no mistakes.
Each test was replicated two times.
Each measure was taken two times.
Tap each cup three times".
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com