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Writing and optimizing distributed-memory kernels for each transposition and contraction is tedious since the number of contractions scales combinatorially with the number of tensor indices.
24 colonies were analyzed from each transposition experiment.
Each transposition reaction was filled up to 3 μg with pEGFP-C1 plasmid (Clontech).
Each transposition event leads to a 15 bp insertion into the TEM-1 gene encoding for a random pentapeptide cassette.
Hence, amplification of MLE lineages occurs regularly, with each transposition burst eventually replaced by another one from another lineage.
For each transposition, 24 plasmids were isolated from individual colonies using the Millipore Montage miniprep system (Millipore, Bedford, USA).
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Each complete transposition cycle produces a new copy.
Each complete transposition cycle produces a new copy of the TE.
When only one strand is cut on each side, the transposition is said to be replicative.
Our result strongly suggested that the gene fragments captured by helitrons happened in sequential fashion, with each step of transposition likely capturing one gene fragments.
Unless stated otherwise, each 50 µl transposition reaction contained 6.7 nM of plasmid substrate and 20 nM transposase in 20 mM Tris-HCl pH8, 100 mM NaCl, 2 mM DTT, 2.5 mM MgCl2 and 10% glycerol.
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