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The peak heat release rate for each test was determined using the model equation based on oxygen depletion index and concentrations of gaseous species such as oxygen, carbon monoxide, carbon dioxide and water.
The elongation during each test was determined by optical measurement (video extensometer) of the displacement of two marker points placed along the waist of the tensile test sample.
The concentration of 4-CP in each test was determined through a UV Vis spectrophotometer (Hach, DR 5000, USA) at λmax = 279 nm.
Responsiveness of each test was determined with calculation of the effect size.
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Using the IFA as the gold standard, the relative sensitivity and specificity of each test were determined.
Cut-points for maximising the sensitivity and specificity for each test were determined using the Youden index.
The amount of staff time, consumable supplies and equipment quantities utilized for each test were determined through direct observation of testing procedures, and included costs associated with quality assurance and quality control.
The geometric mean from each Kruskal Wallis test was determined to be statistically different by applying the multiple mean rank comparison.
The number of bacterial cells in the test was determined for each strain before the test by spreading the appropriate dilutions of bacterial culture on antibiotic-containing LB agar plates and counting the colony forming units after 24 hours growth at 30°C.
Possible cutoff points for the other continuous variables for each laboratory test were determined according to their clinical importance using the results of previous studies and reference values [ 18- 24].
The in vitro minimal fungicidal concentration (MFC) of each compound tested was determined by streaking 10 μl from each well that showed complete inhibition (100% inhibition or a clear well), from the last positive well (growth similar to that of the growth control well), and from the growth control well onto YPD plates.
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